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Useful validation of sorted fractions was carried out by xenotransplantation, the result of which was coupled with miRNA expression profiling to make stem cell-similar miRNA expression profiles.
To find out no matter if miRNA are differentially expressed in LSC and HSC, we fractionated 16 AML affected individual samples and 3 lineage-depleted (Lin–) wire blood (CB) samples making use of CD34 and CD38 into 4 populations and subjected Every single to global miRNA profiling; the stem cell information of each fraction was functionally assayed by xenotransplantation (Figures 1A and S1A).
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(J) Proliferation measured by BrdU incorporation assay of CTRL or 126KD transduced 8227 cells in vitro.
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Self-renewal regulators have remarkably parallel features in malignant and normal stem cells, precluding their therapeutic targeting as a consequence of toxicity to normal stem cells. The opposing self-renewal outcomes governed by miR-126 inside of HSC and LSC point out that Inspite of shared stemness determinants, it may be feasible to target therapeutically the networks that especially Regulate LSC as a result of perturbation of miR-126 levels.
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(E) Percent BrdU incorporation into bulk cultures showing proliferation of CTRL and 126OE transduced 8227 cells after some time.
(D) Consultant intracellular movement plots for that detection of CDK3 and pRB Ser807/811. Graph under signifies 3 unbiased intracellular stream experiments for each ailment wherever the suggest fluorescence depth was in comparison. Mean ± SEM; ∗p=
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